I'm afraid that your question doesn't really make much sense. If you want to call a method in the Load event handler then go ahead and call it. There's nothing stopping you.
That said, the code snippet you posted demonstrates setting properties, not calling methods. It's really not clear what you want to do and what your actual problem doing it is.
That said, the code snippet you posted demonstrates setting properties, not calling methods. It's really not clear what you want to do and what your actual problem doing it is.
Here you go 
Is this what you need?
If you want your function to be called when the form1 starts
If you want your function to be called when a button is clicked
Is this what you need?If you want your function to be called when the form1 starts
Public Class Form1
Function Do_Following() 'This is the function we are calling
item1.Enabled = False
item2.Enabled = False
Return Nothing
End Function
Private Sub Form1_Load(sender As Object, e As EventArgs) Handles Me.Load
Do_Following() 'call the function which we created earlier
End Sub
End Class
If you want your function to be called when a button is clicked
Public Class Form1
Function Do_Following() 'This is the function we are calling
item1.Enabled = False
item2.Enabled = False
Return Nothing
End Function
Private Sub Button1_Click(sender As Object, e As EventArgs) Handles Button1.Click
Do_Following() 'call function
End Sub
End Class
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